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primary antibody cd69  (Bioss)


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    Structured Review

    Bioss primary antibody cd69
    The activation of NK pathway in the EE group. (A–B) KEGG pathway for both up- and downregulated genes. (C) IHC staining was used to detect the expression of <t>CD69.</t> (D) The quantitative analysis of CD69 was performed with ImageJ software. (E–G) q-PCR detected the mRNA expression of NK receptors (H60, RAET, ULBP). n = 5, each group. * p < 0.05, t test. SE: standard environment; EE: environmental enrichment; RAET1: retinoic acid early transcript 1; ULBP1: UL16 binding protein 1.
    Primary Antibody Cd69, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibody cd69/product/Bioss
    Average 94 stars, based on 12 article reviews
    primary antibody cd69 - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Environmental Enrichment Exposure Alleviates Geriatric Depressive-Like Symptoms through Regulating Neurogenesis and Neuroinflammation"

    Article Title: Environmental Enrichment Exposure Alleviates Geriatric Depressive-Like Symptoms through Regulating Neurogenesis and Neuroinflammation

    Journal: Environment & Health

    doi: 10.1021/envhealth.4c00186

    The activation of NK pathway in the EE group. (A–B) KEGG pathway for both up- and downregulated genes. (C) IHC staining was used to detect the expression of CD69. (D) The quantitative analysis of CD69 was performed with ImageJ software. (E–G) q-PCR detected the mRNA expression of NK receptors (H60, RAET, ULBP). n = 5, each group. * p < 0.05, t test. SE: standard environment; EE: environmental enrichment; RAET1: retinoic acid early transcript 1; ULBP1: UL16 binding protein 1.
    Figure Legend Snippet: The activation of NK pathway in the EE group. (A–B) KEGG pathway for both up- and downregulated genes. (C) IHC staining was used to detect the expression of CD69. (D) The quantitative analysis of CD69 was performed with ImageJ software. (E–G) q-PCR detected the mRNA expression of NK receptors (H60, RAET, ULBP). n = 5, each group. * p < 0.05, t test. SE: standard environment; EE: environmental enrichment; RAET1: retinoic acid early transcript 1; ULBP1: UL16 binding protein 1.

    Techniques Used: Activation Assay, Immunohistochemistry, Expressing, Software, Binding Assay



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    The activation of NK pathway in the EE group. (A–B) KEGG pathway for both up- and downregulated genes. (C) IHC staining was used to detect the expression of <t>CD69.</t> (D) The quantitative analysis of CD69 was performed with ImageJ software. (E–G) q-PCR detected the mRNA expression of NK receptors (H60, RAET, ULBP). n = 5, each group. * p < 0.05, t test. SE: standard environment; EE: environmental enrichment; RAET1: retinoic acid early transcript 1; ULBP1: UL16 binding protein 1.
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    Representative tissue images stained using multiplex immunofluorescence staining for CRTH2 (red), Siglec8 (orange), MBP (yellow), <t>CD69</t> (green), and DAPI (blue) in rNP group, Non-rNP group, and control group. White Arrows indicated eosinophils that expressed the corresponding markers. C, control group.
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    Image Search Results


    The activation of NK pathway in the EE group. (A–B) KEGG pathway for both up- and downregulated genes. (C) IHC staining was used to detect the expression of CD69. (D) The quantitative analysis of CD69 was performed with ImageJ software. (E–G) q-PCR detected the mRNA expression of NK receptors (H60, RAET, ULBP). n = 5, each group. * p < 0.05, t test. SE: standard environment; EE: environmental enrichment; RAET1: retinoic acid early transcript 1; ULBP1: UL16 binding protein 1.

    Journal: Environment & Health

    Article Title: Environmental Enrichment Exposure Alleviates Geriatric Depressive-Like Symptoms through Regulating Neurogenesis and Neuroinflammation

    doi: 10.1021/envhealth.4c00186

    Figure Lengend Snippet: The activation of NK pathway in the EE group. (A–B) KEGG pathway for both up- and downregulated genes. (C) IHC staining was used to detect the expression of CD69. (D) The quantitative analysis of CD69 was performed with ImageJ software. (E–G) q-PCR detected the mRNA expression of NK receptors (H60, RAET, ULBP). n = 5, each group. * p < 0.05, t test. SE: standard environment; EE: environmental enrichment; RAET1: retinoic acid early transcript 1; ULBP1: UL16 binding protein 1.

    Article Snippet: The protocol was similar to the IF staining: (1) dewax brain paraffin sections (4 μm) with water; (2) repair antigen and freshen with PBS for 5 min; (3) seal with 5% goat serum for 50 min; (4) add primary antibody CD69 (bs-2499R, Bioss, diluted with 1:200) overnight at 4 °C and wash with PBS for 5 min; (5) add HRP labeled goat antirabbit secondary antibody (GB23303, Servicebio, diluted with 1:200); (6) restain nuclei with DAPI for 10 min; (7) quench tissue autofluorescence; (8) seal sheet.

    Techniques: Activation Assay, Immunohistochemistry, Expressing, Software, Binding Assay

    Representative tissue images stained using multiplex immunofluorescence staining for CRTH2 (red), Siglec8 (orange), MBP (yellow), CD69 (green), and DAPI (blue) in rNP group, Non-rNP group, and control group. White Arrows indicated eosinophils that expressed the corresponding markers. C, control group.

    Journal: Frontiers in Immunology

    Article Title: Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps

    doi: 10.3389/fimmu.2022.1046426

    Figure Lengend Snippet: Representative tissue images stained using multiplex immunofluorescence staining for CRTH2 (red), Siglec8 (orange), MBP (yellow), CD69 (green), and DAPI (blue) in rNP group, Non-rNP group, and control group. White Arrows indicated eosinophils that expressed the corresponding markers. C, control group.

    Article Snippet: At room temperature, we incubated primary antibodies for major basic protein (MBP) (1:150; Boster, China), Siglec8 (1:30; Biolegend, USA), CRTH2 (1:150; Abcam, China), CD69 (1:150; Santa Cruz Biotechnology, China) for 2 hours.

    Techniques: Staining, Multiplex Assay, Immunofluorescence